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Immune cell isolation for flow cytometry Spinal cord tissue was collected after transcardial PBS perfusion and dissociated into single-cell suspensions using 1 mg ml −1 collagenase A (Roche, 11088793001) and 0.1 mg ml −1 DNase I from bovine pancreas (Merck Millipore, 260913) with the gentleMACS Octo Dissociator (Miltenyi Biotec, program: Multi_F)
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186 Further results showed that the UCH domain of USP20 interacts with the N-terminal of SLC7A11, stabilizing SLC7A11 levels by removing the polyubiquitination
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