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Selenium, a Micronutrient That Modulates Cardiovascular Health via Redox Enzymology

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(4)制备Fmoc-Ala-Gly-Leu-Val-树脂： (4) Preparation of Fmoc-Ala-Gly-Leu-Val-resin: 在步骤(3)的Fmoc-Gly-Leu-Val-树脂中，加入脱帽试剂10～50℃反应5～30分钟，用真空泵抽干，重新加入脱帽试剂10～50℃反应10～60分钟，抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，加入用DMF溶解的Fmoc-Ala-OH、DIEA、TBTU/HOBt或HBTU/HOBt或BOP/HOBt的混合物，10～50℃反应10～60分钟，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Ala-Gly-Leu-Val-树脂； In the Fmoc-Gly-Leu-Val-resin of step (3), add decapping reagent 10～50 ℃ and react for 5～30 minutes, use vacuum pump to dry, add decapping reagent 10～50 ℃ and react for 10～60 minutes, pump Dry, wash 3 times with isopropanol, 3 times with DMF, 2 times with redistilled DMF, add a mixture of Fmoc-Ala-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF , reacted at 10-50°C for 10-60 minutes, pumped dry with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with distilled DMF, and dried to obtain Fmoc-Ala-Gly-Leu- Val-resin