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Assessment of binding and release of captured molecules to/from loaded magnetic beads To evaluate the capturing capacity of loaded beads, different amounts of APC (2.5 µg, 5 µg, 10 µg and 20 µg) were dissolved in 500 µl binding buffer and incubated with loaded beads under a shaking speed of 1,100 rpm at RT
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Size:500 mg / 30 capsules
Finally, EVs were obtained by centrifugation at 100,000 × g , followed by filtration through 0.22 μm or 0.45 μm pore-size membranes to enhance purity
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