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In separate wells, either Alexa Fluor™ 488 AcLDL (Thermo Fisher, L23380) or FITC-FSA (Gift from Karen Sørensen), both at 2 µg/ml, was added to the culture medium

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Using in silico design, structure–activity relationships (SARs), chemical optimization, cell-based assays and in vivo experiments, we identified and characterized the first-in-kind small-molecule inhibitors of STX7–Munc13-4-dependent endosomal maturation and signaling for use in immune cells that contribute to systemic inflammation