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The labeling solution was removed and replaced by an ice-cold solution of 100 mM of glycine for 10 min at 4 °C.Cells were washed two times with ice cold PBS and lysed in a RIPA buffer containing a cocktail of protein phosphatase (#B15001-A and B15001-B, Bimake) and protease inhibitors (#B14001, Bimake) at 1x
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