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Because STI1 in yeast is a co-chaperone that preferentially regulates kinases [17], we cannot exclude the possibility that it may also directly regulate kinase activity, including PLK3
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Then, primary antibody binding was detected after incubating sections with a horseradish peroxidase conjugated-secondary antibody (polyclonal anti-goat or anti-rabbit IgG HRP, R&D System, Minneapolis, MN, USA) for 2.5 h, followed by a 20 min exposure to the HRP substrate 3-amino-9-ethylcarbazole (AEC)
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