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aeruginosa strains were grown in glycerol alanine minimal medium (GA medium) containing 1% glycerol, 67.3 mM L-alanine, 8 mM MgSO 4 , 0.44 mM K 2 HPO 4 , and 0.065 mM FeSO 4 at 37 °C for 24 h, and pyocyanin production was quantified based on the absorbance of pyocyanin at 520 nm (OD 520nm ) in an acidic solution as describe previously [61]

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This suggests that the neuronal gene dysregulation and immune cell infiltration, while co-occurring in AD, may represent parallel pathological processes or interact through complex, non-linear mechanisms not captured by bulk-tissue correlation analysis