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The bacterial pellet was lysed using a sonicator (Sonics VCX-750-110) on ice for 3 min (2 s on, 2 s off, and 60% amplitude) in a buffer containing 50 mM HEPES pH 7.4, 150 mM NaCl, 10 mM Dithiothreitol (DTT), 12% (v/v) glycerol, a protease inhibitor tablet (Roche 05056489001) and 1 mM PMSF
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Focus groups worked on refining the wording and conceptual clarity of the survey (Groves et al., 2009)
essential amino acid that helps to maintain overall good health by facilitating the transfer of fatty acid groups into the mitochondrial membrane for cellular energy production
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