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To study whether neonatal intestinal macrophages promote endothelial cell sprouting in vitro, red-fluorescent intestinal endothelial cells [isolated from neonatal mT/mG mouse lamina propria (LP)] and CD11b + myeloid cells (from neonatal Cx3cr1 -GFP reporter mouse LP) were cultured separately or together in Matrigel with growth factor-containing culture media (LL-0005, Lifeline Cell Technology) and endothelial cell sprouting was assessed (Fig

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