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[DOI] [PMC free article] [PubMed] [Google Scholar] Gouspillou G, Hepple RT (2013) Facts and controversies in our understanding of how caloric restriction impacts the mitochondrion

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(3)制备Fmoc-Gly-Leu-Val-WangResin (3) Preparation of Fmoc-Gly-Leu-Val-WangResin 在步骤(2)的Fmoc-Leu-Val-WangResin中，加入脱帽试剂(20％哌啶的DMF溶液)，常温反应5分钟，抽干，重新加入脱帽剂常温反应20分钟，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，加入Fmoc-Gly-OH0.7g，DIEA0.4ml，TBTU0.56g，HOBT0.2g，DMF10ml常温反应1.5小时，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Gly-Leu-Val-WangResin； In the Fmoc-Leu-Val-WangResin of step (2), add decapping reagent (DMF solution of 20% piperidine), react at room temperature for 5 minutes, drain, add decapping reagent at room temperature and react for 20 minutes, wash with isopropanol 3 times, washed 3 times with DMF, washed 2 times with re-distilled DMF, drained, added Fmoc-Gly-OH0.7g, DIEA0.4ml, TBTU0.56g, HOBT0.2g, DMF10ml, reacted at room temperature for 1.5 hours, pumped with a vacuum pump Dry, wash 3 times with isopropanol, wash 3 times with DMF, wash 2 times with DMF that has been distilled, and drain to obtain Fmoc-Gly-Leu-Val-WangResin