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Outdoor winter training, cold water access in lakes and rivers during spring and fall, and simply spending time in subzero temperatures activates similar physiological responses to dedicated cryotherapy chambers

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a modified mutant human NNMT [lacking 3 amino acid residues from the C-terminus of the NNMT protein that was not observed in crystal structure] (3ROD, PDB accession code) cloned into an IPTG-inducible plasmid pJ401 expression vector was purchased from DNA 2.0 (Menlo Park, Calif.)