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Additionally, when BMECs were challenged with gamma-d-glutamyl-meso-diaminopimelic acid (iE-DAP), a component of bacterial cell walls, it induced inflammatory changes and oxidative stress, which were effectively mitigated by arginine and methionine treatment (230)
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The supernatant was incubated overnight with 3 μg of the appropriate antibody with rotation and precipitated by the addition of 30 μl of protein-A/G beads at 4 °C and mixing for an additional 3 h
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