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Thereafter, DTT and EDTA were removed by passing the sample through a PD-10 column (Cytiva) pre-equilibrated with Chelex-treated and degassed reaction buffer (50 mM HEPES-NaOH, pH 7.2, and 150 mM NaCl)
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And overall my goal was not to do anything and just enhance the body's own ability to to defend itself
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