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Construct Design Considerations For researchers designing expression constructs, platforms like Orbion can help identify potential problems before cloning: Secondary cleavage site detection: Identify sequences that match protease recognition patterns Terminus accessibility analysis: Predict whether N- and C-termini are accessible or structured PTM site mapping: Ensure cleavage sites don't overlap with essential modifications Disorder prediction: Identify flexible regions suitable for tag placement Good construct design prevents most tag removal problems—and good design starts with understanding your protein's structure and sequence features before you order the first oligonucleotide

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