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The immunoblots were visualized using ECL Western blotting substrate (Pierce Biotechnology) on a Chemidoc XRS imager (Bio Rad) or using Dylight 800-conjugated secondary antibodies (Thermo Fisher Scientific, Rockford, IL) on an Odyssey Imaging Systems (LI-COR Biosciences, Lincoln, NE)

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Irrelevance of Alkaline pH in ERW Importantly, although ERW has an alkaline pH [1,71,72], we can logically exclude this property from being a contributing factor to its therapeutic effects because, in addition to the facts mentioned above, in all the in vitro cell culture studies, the alkaline pH is always neutralized [29,33,35,40,43,44,73,74,75,76,77,78,79,80,81,82]