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The immunostained sections were scanned on a NanoZoomer NDP system (Hamamatsu Photonics, Shizuoka, Japan) with 20 × resolution, or on a FV3000 confocal microscope (Olympus, Tokyo, Japan) with 4, 10 or 20 × resolution
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When particles are of similar sizes or below 200 nm, making them difficult to monitor clearly, fluorescence staining can be employed to distinguish them in real time
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