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The lysate was subsequently centrifuged (12000 × g, 4 ºC, 5 min), denatured, analysed by SDS‒PAGE, and transferred to PVDF membranes (IPVH15150, Millipore)

HSA

Net Volume:Kit

The intestines regulate Mn through both absorption and excretion, which is controlled by specific transporters localized on the apical (lumen-facing) and basolateral (blood-facing) membranes of the intestinal epithelium (Figure 1A)