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2.2 Preparation of mouse embryos Embryos were prepared by in vitro fertilization (IVF) based on our previous reports ( 2.3 Generation of Tle6 KO mice using CRISPR-Cas9 system To generate Tle6 KO mice, crRNAs were designed using CRISPR-direct ( ( Information regarding the primers used for PCR genotyping is provided in Supplementary Table S1

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