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ECs were treated with IL-1β (100 ng/ml) for 24 h with or without 50 μM ferroptosis inhibitor ferrostatin-1 or 10 μM ferroptosis activator erastin at 37°C, rinsed with PBS, fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 (Beyotime Institute of Biotechnology) for 5 min at 25°C

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