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To generate fusion proteins consisting of GST or a Strep-tag fused to piUL49 with a leucine substituted for Arg-83, pEu-GST-iUL49R83L, or pcDNA-SE-iUL49R83L, respectively, were constructed by amplifying the entire coding sequences of piUL49R83L from iUL49R83L genome by PCR, and cloning it into pEu-E01-GST-TEV-MCS-N01 and pcDNA-SE in frame with GST and a Strep-tag, respectively
b5
Color:Denim
Fierbinteanu-Braticevici, C
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