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Extracellular glutamate levels are tightly maintained at low concentrations during physiologic conditions mainly by glial EAATs
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Cell staining and flow cytometric analysis Cell surface staining of single-cell suspensions was performed using fluorescently conjugated antibodies in combination with pMHCI tetramers L d -IE1 168โ176 , or D b -M45 985โ993 for the identification of MCMV-specific CD8 T cells, D b -NP 366โ374 for the identification of IAV-specific CD8 T cells, or K b -OVA 257โ264 for the identification of OVA-specific CD8 T cells
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